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| Gonzalez-fernandez, Carmen; Tallec, Kevin; Le Goic, Nelly; Lambert, Christophe; Soudant, Philippe; Huvet, Arnaud; Suquet, Marc; Berchel, Mathieu; Paul-pont, Ika. |
| While the detection and quantification of nano-sized plastic in the environment remains a challenge, the growing number of polymer applications mean that we can expect an increase in the release of nanoplastics into the environment by indirect outputs. Today, very little is known about the impact of nano-sized plastics on marine organisms. Thus, the objective of this study was to investigate the toxicity of polystyrene nanoplastics (NPs) on oyster (Crassostrea gigas) gametes. Spermatozoa and oocytes were exposed to four NPs concentrations ranging from 0.1 to 100 mg L−1 for 1, 3 and 5 h. NPs coated with carboxylic (PS-COOH) and amine groups (PS-NH2) were used to determine how surface properties influence the effects of nanoplastics. Results demonstrated the... |
| Tipo: Text |
Palavras-chave: Nanoplastics; Oysters; Gametes; Cellular responses. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00443/55459/56976.pdf |
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| González-fernández, Carmen; Toullec, Jordan; Lambert, Christophe; Le Goïc, Nelly; Seoane, Marta; Moriceau, Brivaela; Huvet, Arnaud; Berchel, Mathieu; Vincent, Dorothée; Courcot, Lucie; Soudant, Philippe; Paul-pont, Ika. |
| The potential presence of nanoplastics (NP) in aquatic environments represents a growing concern regarding their possible effects on aquatic organisms. The objective of this study was to assess the impact of polystyrene (PS) amino-modified particles (50 nm PSNH2) on the cellular and metabolic responses of the diatom Chaetoceros neogracile cultures at two essential phases of the growth cycle, i.e. exponential (division) and stationary (storage) phases. Both cultures were exposed for 4 days to low (0.05 μg mL−1) and high (5 μg mL−1) concentrations of PS-NH2. Exposure to NP impaired more drastically the major cellular and physiological parameters during exponential phase than during the stationary phase. Only an increase in ROS production was observed at... |
| Tipo: Text |
Palavras-chave: Nanoplastics; Diatoms; Physiology; Transparent exopolymer particles. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00491/60235/63627.pdf |
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| Tallec, Kevin; Huvet, Arnaud; Di Poi, Carole; Gonzalez-fernandez, Carmen; Lambert, Christophe; Petton, Bruno; Le Goic, Nelly; Berchel, Mathieu; Soudant, Philippe; Paul-pont, Ika. |
| In the marine environment, most bivalve species base their reproduction on external fertilization. Hence, gametes and young stages face many threats, including exposure to plastic wastes which represent more than 80% of the debris in the oceans. Recently, evidence has been produced on the presence of nanoplastics in oceans, thus motivating new studies of their impacts on marine life. Because no information is available about their environmental concentrations, we performed dose-response exposure experiments with polystyrene particles to assess the extent of micro/nanoplastic toxicity. Effects of polystyrene with different sizes and functionalization (plain 2-μm, 500-nm and 50-nm; COOH-50 nm and NH2-50 nm) were assessed on three key reproductive steps... |
| Tipo: Text |
Palavras-chave: Oyster; Embryos; Gametes; Microplastics; Nanoplastics. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00453/56419/58090.pdf |
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| Tallec, Kevin; Blard, Océane; González-fernández, Carmen; Brotons, Guillaume; Berchel, Mathieu; Soudant, Philippe; Huvet, Arnaud; Paul-pont, Ika. |
| Plastic debris are classified as a function of their size and recently a new class was proposed, the nanoplastics. Nano-sized plastics have a much greater surface area to volume ratio than larger particles, which increases their reactivity in aquatic environment, making them potentially more toxic. Only little information is available about their behavior whereas it crucially influences their toxicity. Here, we used dynamic light scattering (DLS) to explore the influence of environmental factors (fresh- and saltwater, dissolved organic matter) on the behavior (surface charge and aggregation state) of three different nano-polystyrene beads (50 nm), with (i) no surface functionalization (plain), (ii) a carboxylic or (iii) an amine functionalization. Overall,... |
| Tipo: Text |
Palavras-chave: Nanoplastic; Dynamic light scattering; Behavior; Aggregation; Salinity; Organic matter. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00485/59652/62684.pdf |
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